Expression of isoleucine zipper modified soluble CD40L in Pichia pastoris / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 844-847, 2006.
Article
de Zh
| WPRIM
| ID: wpr-320469
Bibliothèque responsable:
WPRO
ABSTRACT
To obtain the expression of Isoleucine Zipper modified soluble CD40L (IZ-sCD40L) in Pichia pastoris, firstly, DNA fragment of IZ-sCD40L was obtained by PCR and over-lap PCR . Then the expression vector pPICZaA-IZ-sCD40L was constructed. Nucleotide sequencing analysis indicated that the DNA fragment of IZ-sCD40L was correctly inserted into the pPICZaA vector. Linearized pPICZ(alpha)A-IZ-sCD40L was introduced into Pichia pastoris GS115. Positive clone was selected by PCR and its phenotype was determined. The positive clone was introduced with methanol. The results of SDS-PAGE and Western blot showed that product was recombinant Isoleucine Zipper modified soluble CD40L fusion protein.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pichia
/
Plasmides
/
Recombinaison génétique
/
Protéines de fusion recombinantes
/
Angiotensine-II
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Réaction de polymérisation en chaîne
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Clonage moléculaire
/
Ligand de CD40
/
Génétique
/
Isoleucine
langue:
Zh
Texte intégral:
Journal of Biomedical Engineering
Année:
2006
Type:
Article