Isolation and in vitro characterization of CD133(+) side population cells from laryngeal cancer cell line / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
;
(12): 752-757, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-322478
ABSTRACT
<p><b>OBJECTIVE</b>To investigate an approach enriching cancer stem cells (CSCs) more effectively from laryngeal cancer cell line.</p><p><b>METHODS</b>CD133(+)SP and CD133(-)SP subpopulation was detected and isolated from Hep-2 cell line using Hoechst33342 dye and phycoerythrin (PE)-conjugated CD133 monoclonal antibody assisted by fluorescence activated cell sorting technology. Sorted CD133(+)SP and CD133(-)SP cells were compared in CSCs-related assays including proliferation, differentiation, spheroid formation and drug sensitivity.</p><p><b>RESULTS</b>CD133(+)SP cells accounted for a very small fraction of (0.30 ± 0.12)% in Hep-2 cell line, far less than the proportion of CD133(+) subgroup and side population subgroup, which were (3.15 ± 0.83)% and (17.1 ± 2.0)% respectively. Intriguingly, CD133(+)SP cells proliferated much faster than CD133(-)SP cells in RPMI1640 and gave rise to CD133(-)SP cells and other heterogeneous cells that formed the bulk of the tumor. In contrast, CD133(-)SP cells were not able to differentiate into CD133(+)SP cells. In serum-free medium CD133(+)SP cells grew as spherical clusters and remained floating. In addition, CD133(+)SP cells manifested the marked resistance to chemotherapy than CD133(-)SP cells.</p><p><b>CONCLUSIONS</b>Compared with CD133(-)SP cells, CD133(+)SP subpopulation exhibited extraordinary cancer stem-like properties, were enriched for cancer stem cells more effectively and might serve as an ideal putative candidate for CSCs research in laryngeal cancer.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Peptides
/
Cellules souches tumorales
/
Glycoprotéines
/
Antigènes CD
/
Différenciation cellulaire
/
Séparation cellulaire
/
Tumeurs du larynx
/
Biologie cellulaire
/
Lignée cellulaire tumorale
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Année:
2011
Type:
Article
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