Preparation and characterization of mono PEGylated recombinant human interferon omega / 药学学报
Acta Pharmaceutica Sinica
; (12): 393-398, 2012.
Article
Dans Zh
| WPRIM
| ID: wpr-323030
Responsable en Bibliothèque :
WPRO
ABSTRACT
The amino group PEGylation of rhIFNomega with monomethoxy polyethylene glycol succinimidyl succinate (mPEG-SS, 20 000) was investigated, and the modified mixture was separated and purified by ion exchange chromatography and gel filtration chromatography. Under the optimized purification conditions, the average content ofmono PEG-rhIFNomega in the collect liquid reached 182 microg x mL(-1). The average purified yield of mono PEG-rhIFNomega exceed to 22%, and the purity of mono PEG-rhIFNomega was greater than 98% by SDS-PAGE and RP-HPLC. Relative molecular mass of mono PEG-rhIFNomega was 43 790 detected by MALDI-TOF MS. The apparent molecular mass measured by SDS-PAGE was about 60 810. The purified PEG-rhIFNomega has the characteristics of typical PEGylated protein. Activity reservation rate of mono PEG-rhIFNomega was 15.0%, while the antigenicity decreased by at least 64 folds. In addition, the acid stability, thermal stability and stability in serum and trypsin solution of mono PEG-rhIFNomega were markedly better than those of the rhIFNomega. The pharmacological properties of mono PEG-rhIFNomega were significantly improved. The prepared PEG-rhIFNomega might be developed to a novel safe and long-acting interferon.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Polyéthylène glycols
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Protéines recombinantes
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Interféron de type I
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Chimie
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Chromatographie sur gel
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Chromatographie en phase liquide à haute performance
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Chromatographie d'échange d'ions
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Spectrométrie de masse MALDI
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Stabilité de médicament
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Électrophorèse sur gel de polyacrylamide
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2012
Type:
Article