Culture and identification of human adult Leydig cells / 中华男科学杂志
National Journal of Andrology
;
(12): 356-358, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-323360
ABSTRACT
<p><b>OBJECTIVE</b>To obtain a simple and effective method to isolate and purify adult Leydig cells.</p><p><b>METHODS</b>The testes of human adults were digested and then the density gradient centrifugation of the cells was performed with four different Percoll concentrations (60%, 34%, 26%, 21%) to isolate Leydig cells, whose characteristics were identified by cytological observation staining, 3beta-HSD staining and detection of hCG and testosterone secretion.</p><p><b>RESULTS</b>High-concentration (> 90%) purified Leydig cells were acquired, and many identification experiments demonstrated the adequate testosterone secretory function of the isolated and purified Leydig cells.</p><p><b>CONCLUSION</b>This method is easy and efficient for the isolation and purification of adult Leydig cells.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Testostérone
/
Séparation cellulaire
/
Cellules cultivées
/
Povidone
/
Silice
/
Sécrétions corporelles
/
Biologie cellulaire
/
Gonadotrophine chorionique
/
Cellules de Leydig
Type d'étude:
Étude pronostique
Limites du sujet:
Adulte
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
National Journal of Andrology
Année:
2005
Type:
Article
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