Determination of rubrofusarin gentiobioside in Cassia obtusifolia by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 366-368, 2008.
Article
Dans Zh
| WPRIM
| ID: wpr-324283
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for determining the content of rubrofusarin gentiobioside in Cassia obtusifolia.</p><p><b>METHOD</b>The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, acetonitrile-THF-1% acetic acid (18: 3:79) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees 2 and detection wavelength at 278 nm.</p><p><b>RESULT</b>A good linearity was obtained from 0.1-0.5 microg with r = 0.999 9 for rubrofusarin gentiobioside. The average recovery was 101.1%, and RSD was 2.23% (n = 5).</p><p><b>CONCLUSION</b>The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Médicaments issus de plantes chinoises
/
Cassia
/
Chimie
/
Reproductibilité des résultats
/
Chromatographie en phase liquide à haute performance
/
4H-1-Benzopyran-4-ones
/
Glucosides
/
Méthodes
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2008
Type:
Article