Effects of puerarin on expression of nuclear factor kappaB after cerebral ischemia/reperfusion in rats / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2515-2518, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-324337
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the activation of nuclear factor kappaB (NF-KB) and the influence of puerarin on it after cerebral ischemia-reperfusion in rats.</p><p><b>METHOD</b>Cerebral ischemia-reperfusion injury was induced by 90 min of middle cerebral artery (MCA) occlusion and followed by 2, 6, 12, 24, 72 h reperfusion. Puerarin or saline was intra-peritoneally injected 1h before MCA occlusion and then the drugs were administered once every six hours. The infarct volume and brain edema were determined by TTC stain. Level of NF-kappaB P65 subunit was determined by immunohistochemistry and western blot.</p><p><b>RESULT</b>Immunohistochemistry revealed the translocation of NF-kappaB. A time course of NF-kappaB induction in brain showed that NF-kappaB P65 subunit obviously increased at 6 h, peaked at 24 h and then decreased by 72 h post-reperfusion. Puerarin decreased the level of NF-kappaB at 24, 72 h after reperfusion. There was a decrease trend in brain infarct volume between puerarin and control.</p><p><b>CONCLUSION</b>NF-kappaB is translocated and its level is increased after ischemia-reperfusion. Puerarin may attenuate the ischemia-reperfusion injury through inhibition of NF-kappaB activation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Plantes médicinales
/
Encéphale
/
Immunohistochimie
/
Lésion d'ischémie-reperfusion
/
Répartition aléatoire
/
Chimie
/
Technique de Western
/
Rat Sprague-Dawley
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2007
Type:
Article
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