Kinetics of enzymatic hydrolysis of steam-explosion pretreated corn straw / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 592-597, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-324524
ABSTRACT
In order to learn the enzymatic hydrolysis characteristics of steam-explosion pretreated corn straw by cellulase, the effects of substrate concentration, cellulase concentration and temperature were determined. The kinetics of the hydrolysis reaction could be described with the Michealis-Menten equation, and the hydrolysis reaction obeyed the classical first-order reaction rate in the first three hours. In the condition of 45 degrees C and pH 5.0 and the stirring rate 120 r/min, the Michealis constant (Km) and maximum rate (Vm) for 1.2 FPU/mL of cellulase were 11.71 g/L and 1.5 g/(L x h). The kinetic model, including the parameters such as substrate concentration, enzymatic concentration and temperature, was suit for the hydrolysis reaction under the temperature range from 30 degrees C-50 degrees C.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Vapeur
/
Cinétique
/
Catalyse
/
Cellulase
/
Chimie
/
Tiges de plante
/
Zea mays
/
Hydrolyse
Type d'étude:
Étude pronostique
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2011
Type:
Article
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