Study on the molecular epidemiology of beta-lactamase TEM gene in isolated Streptococcus pneumoniae / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 970-972, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-324979
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the beta-lactamase TEM gene of isolated Streptococcus pneumoniae (Sp) in Suzhou area.</p><p><b>METHODS</b>Twenty-three strains of Sp were collected from respiratory tract secretions of children with respiratory diseases in Nov 2002 to Apr 2003 at Children's Hospital of Suzhou University (reference strain ATCC49619) to build TEM polymerase chain reaction (PCR) system (reference strain E. coli. 9-j53R1 with TEM gene) TEM gene of 23 strains was detected to comparo the sequences with published TEM gene sequences in GenBank for analyzing TEM gene model.</p><p><b>RESULTS</b>Twenty-one strains had TEM gene with a positive rate of 91.3% (21/23). TEM-129 gene were confirmed from No.17 (SR017, penicillin resistance) TEM sequence. New discovered TEM-129 sequence had a modification (ATG[M]-->ATA[I]) at No.182 code and published (GenBank www.ncbi.nlm.nih.gov/nucleotide, AY452662). TEM-1 genes were confirmed from other TEM sequences. New discovered TEM-1 gene of isolated Sp had been published (GenBank www.ncbi.nlm.nih.gov/nucleotide, AY392531) too.</p><p><b>CONCLUSION</b>Isolated Sp had TEM gene (TEM-129, EM-1 genotype) with a positive rate of 91.3%. The result enriched the understanding of isolated Sp with penicillin resistance.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Streptococcus pneumoniae
/
Bêta-Lactamases
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Chine
/
Épidémiologie
/
Mutation ponctuelle
/
Épidémiologie moléculaire
/
Pneumopathie bactérienne
/
Résistance aux bêta-lactamines
Type d'étude:
Étude de dépistage
Limites du sujet:
Femelle
/
Humains
/
Mâle
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Epidemiology
Année:
2004
Type:
Article
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