Comparative study on in vitro stationary culture system or superfusion culture system of cow mammary tissue / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1040-1046, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-325428
ABSTRACT
Dairy cow mammary tissue was cultured in superfusion system or stationary system, and the influence of these two methods in the activity and ultrastructure of tissue was investigated according to LDH vigor, trypan blue dying, agrose gel electrophoresis, transmission microscope observation. The results showed that the mammary tissue cultured in superfusion system could keep normal tissue activity and ultrastructure within 12-60 h in DMEM plus 10% calf serum, while mammary tissue stationary culture could keep normal tissue activity and ultrastructure within 60-108 h. Both culture systems had some advantages and disadvantages.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Perfusion
/
Facteurs temps
/
Cinétique
/
Microscopie électronique
/
Bioréacteurs
/
Techniques de culture de tissus
/
Glandes mammaires animales
/
Méthodes
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2006
Type:
Article
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