Study on effect of enzyme linked immunosorbent assay kits of norovirus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 227-228, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-325583
ABSTRACT
<p><b>OBJECTIVE</b>Effects of RIDASCREEN Norovirus (C 1401) 3rd Generation kit (R-biopham AG, darmstadt, Germany) and IDEIA NLV kit (DAKOCytomation., Ely, UK) were compared for detecting human norovirus (HuNV) in fecal sample.</p><p><b>METHODS</b>The performance of the ELISA was compared with that of the reverse transcription polymerase chain reaction (RT-PCR) by testing a panel of 308 fecal samples collected from patients involved in outbreaks of gastroenteritis in Chang Chun and Guang Zhou. Gene sequencing was performed to positive samples tested by RT-PCR to determine genotype compared with standard sequences.</p><p><b>RESULTS</b>RT-PCR is gold standard, RIDASCREEN Norovirus (C 1401) 3rd Generation kit had a high sensitivity of 96.10% but a specificity of 93.51%, and Dako kit had a low sensitivity of 95.83% but a high specificity of 95.76%.</p><p><b>CONCLUSION</b>RIDASCREEN Norovirus (C 1401) 3rd Generation kit is more Satisfactory for a preliminary screening.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Trousses de réactifs pour diagnostic
/
Virologie
/
Test ELISA
/
Chimie
/
Épidémies de maladies
/
Infections à Caliciviridae
/
Norovirus
/
Diagnostic
/
Allergie et immunologie
/
Fèces
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2009
Type:
Article
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