Prevention of bone loss by injection of insulin-like growth factor-1 after sciatic neurectomy in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 158-162, 2013.
Article
Dans Anglais
| WPRIM
| ID: wpr-325720
ABSTRACT
<p><b>OBJECTIVE</b>Injection of insulin-like growth factor-1 (IGF-1) can prevent bone loss in sciatic nerve transaction rats. We try to investigate the action mechanism of IGF-1 on bone formation.</p><p><b>METHODS</b>A total of 40 adult male Spragne-Dawley rats were divided into two groups (experimental group and control group) with 20 animals in each. Sciatic neurectomy was performed to model disuse osteoporosis in all rats. IGF-1 was administered in experimental group with the dose of 100 microgramme/kilogram per day for 3 days. Meanwhile, the rats in control group were treated with saline. Bone mineral density was measured by dual-energy X-ray absorptiometry 4 and 6 weeks after neurectomy respectively. Expression of Osterix and Runx2 was determined by reverse transcription-polymerase chain reaction (RT-PCR) assay.</p><p><b>RESULTS</b>There was a significant increase in the bone mineral density of experimental group compared with control group. There was a significant decrease in the level of receptor activator of nuclear factor-kappaB-ligand but an increase in the level of osteoprotegerin 4 and 6 weeks after neurectomy in the experimental group compared with control one. The expression of Osterix and Runx2 was up-regulated in the bone marrow of experimental group compared with control group.</p><p><b>CONCLUSION</b>IGF-1 can increase bone formation by stimulation of osteoblast number and activity, and reduce bone resorption by restriction of differentiation of osteoclast, suggesting that IGF-1 may improve the therapeutic efficacy for disuse osteoporosis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoblastes
/
Physiologie
/
Nerf ischiatique
/
Chirurgie générale
/
Facteurs de transcription
/
Résorption osseuse
/
Facteur de croissance IGF-I
/
Immunohistochimie
/
Densité osseuse
/
Différenciation cellulaire
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese Journal of Traumatology
Année:
2013
Type:
Article
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