Influence of acute ethanol intoxication on neuronal apoptosis and Bcl-2 protein expression after severe traumatic brain injury in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 136-139, 2013.
Article
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| WPRIM
| ID: wpr-325724
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the influence and mechanism of acute ethanol intoxication (AEI) on rat neuronal apoptosis after severe traumatic brain injury (TBI).</p><p><b>METHODS</b>Ninety-six Sprague-Dawley rats were randomly divided into four groups: normal control, AEI-only, TBI-only and TBI+AEI (n equal to 24 for each). Severe TBI model was developed according to Feeney's method. Rats in TBI+AEI group were firstly subjected to AEI, and then suffered head trauma. In each group, animals were sacrificed at 6 h, 24 h, 72 h, and 168 h after TBI. The level of neuronal apoptosis and the expression of Bcl-2 protein were determined by TUNEL assay and immunohistochemical method, respectively.</p><p><b>RESULTS</b>Apoptotic cells mainly distributed in the cortex and white matter around the damaged area. Neuronal apoptosis significantly increased at 6 h after trauma and peaked at 72 h. Both the level of neuronal apoptosis and expression of Bcl-2 protein in TBI-only group and TBI+AEI group were higher than those in control group (P less than 0.05). Compared with TBI-only group, the two indexes were much higher in TBI+AEI group at all time points (P less than 0.05).</p><p><b>CONCLUSION</b>Our findings suggest that AEI can increase neuronal apoptosis after severe TBI.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Physiologie
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Intoxication
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Lésions encéphaliques
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Immunohistochimie
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Cortex cérébral
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Prosencéphale
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Rat Sprague-Dawley
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Apoptose
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Protéines proto-oncogènes c-bcl-2
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Méthode TUNEL
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
langue:
En
Texte intégral:
Chinese Journal of Traumatology
Année:
2013
Type:
Article