Readspace solid-phase microextraction-gas chromatography for determination of 2,5-hexanedione in urine / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for determination of 2,5-hexanedione in urine by headspace solid-phase microextraction-gas chromatography.</p><p><b>METHODS</b>After extraction by solid-phase microextraction head, 2,5-hexanedione in urine was determined by gas chromatography and was quantified by external standard method.</p><p><b>RESULTS</b>The concentration of 2,5-hexanedione in urine showed a linear relationship within the range of 0.1-20.0 µg/ml. The regression equation was y=261.36x-1.903 3, r=0.999 2. The minimum detectable concentration was 0.01 µg/ml. The recovery rate was 92.6%-97.1%, with a relative standard deviation (RSD) of 3.3%-5.8%. The intra-day and inter-day RSDs were 3.8%-6.2% and 4.7%-6.3% respectively.</p><p><b>CONCLUSION</b>This determination method has no requirement for organic solvents, features simple and rapid operation, possesses higher detection sensitivity, and applies well to the determination of 2,5-hexanedione in urine.</p>