Study on effect of anisodamine on expressions of tissue factor and plasminogen activator-1 inhibitor in vascular endothelial cells and its mechanisms / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 422-426, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-326732
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of anisodamine in treating infectious shock through studying effect of anisodamine on endotoxin lipopolysaccharide (LPS) induced expression of tissue factor (TF) and plasminogen activator inhibitor type 1 (PAI-1) in vascular endothelial cells (EC).</p><p><b>METHODS</b>Human umbilical vein endothelial cells (HUVEC) were cultured by trypsin digestion method. PAI-1 was measured in the conditioned medium of HUVEC by a specific enzyme-linked immunosorbent assay (ELISA), whereas TF activity was measured in the lysates of these cells by using a single step clotting assay. Specific mRNA expressions were determined by Northern blotting. In order to evaluate a possible contribution of the nuclear factor-kappa B (NF-kappa B) pathway on the transductive effects observed, electrophoretic mobility shift assays (EMSA) were performed using nuclear extracts from HUVEC and NF-kappa B binding oligonucleotides.</p><p><b>RESULTS</b>LPS could significantly strengthen the expression of HUVEC PAI-1 protein and TF activity and its mRNA, this effect of LPS could be markedly weakened after adding Anisodamine dose-dependently. Anisodamine could also completely block the LPS induced NF-kappa B DNA binding activity in nuclear extracts from HUVEC.</p><p><b>CONCLUSION</b>The possible mechanism of anisodamine in treating infectious shock may be through antagonizing LPS induced HUVEC TF and PAI-1 expression, and the antagonism might be, at least partially, transduced by path of NF-kappa B.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Alcaloïdes des Solanaceae
/
Veines ombilicales
/
ARN messager
/
Thromboplastine
/
Médicaments issus de plantes chinoises
/
Cellules cultivées
/
Facteur de transcription NF-kappa B
/
Inhibiteur-1 d'activateur du plasminogène
/
Milieux de culture conditionnés
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Integrated Traditional and Western Medicine
Année:
2004
Type:
Article
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