Cloning associated genes using microdissection-cDNA PCR-SSH in gastric dysplasia / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 573-576, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-327034
ABSTRACT
<p><b>OBJECTIVE</b>To construct cDNA subtracted libraries from gastric dysplasia and further screen differentially expressed genes.</p><p><b>METHODS</b>Relatively pure dysplasia and normal tissue were procured by manual microdissection, and amplified by cDNA-PCR, which was used to carry on for suppression subtractive hybridization (SSH). Subtracted cDNA fragments were linked with vector, cloned, screened, sequenced, and made homologous search. Differentially expressed fragments were verified by dot hybridization.</p><p><b>RESULTS</b>Two subtracted cDNA libraries were constructed. Among 26 sequenced clones, 15 fragments corresponded to known genes, 3 fragments were known EST and 8 fragments were unknown EST (GenBank BQ164614-BQ164616, BQ291516-BQ291520). Fifteen fragments were verified to be differentially expressed in gastric dysplasia.</p><p><b>CONCLUSIONS</b>Subtracted cDNA libraries from gastric dysplasia are constructed using combination of microdissection-cDNA PCR and SSH setup in our laboratory. Some fragments have been screened and verified to help to search for novel associated genes with gastric carcinogenesis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
États précancéreux
/
Tumeurs de l'estomac
/
Régulation de l'expression des gènes tumoraux
/
Banque de gènes
/
Réaction de polymérisation en chaîne
/
Clonage moléculaire
/
Analyse de séquence d'ADN
/
ADN complémentaire
/
Analyse de profil d'expression de gènes
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2003
Type:
Article
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