Effects of low shear stress on expression of platelet derived growth factor (PDGF) of organ-cultured artery in vitro / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 5-9, 2005.
Article
Dans Zh
| WPRIM
| ID: wpr-327146
Responsable en Bibliothèque :
WPRO
ABSTRACT
To explore the biological effects of shear stress on intact artery and the change of growth factor during stress-induced vascular remodeling, we established an artery organ-cultured system under stress in vitro, and the common carotid arteries of pigs were cultured under shear stress of 20, 5 and 0 dyn/cm2. PDGF-A synthesis of vascular smooth muscle cells (VSMCs) cultured for 1, 4 and 7 days were studied by immunohistochemical and computer image processing methods, and PDGF-B secretion of endothelial cells (ECs) cultured within 12 h were studied by ELISA. Results showed that PDGF-B increased obviously under shear stress of 5 dyn/cm2, and reached the highest point at about 3 h; PDGF-A synthesis also obviously increased under low shear stress in 7 days. Increasing of PDGF synthesis promotes phenotype switch and proliferation of VSMC. It may have important influence on artery remodeling under low shear stress.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Contrainte mécanique
/
Suidae
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Facteur de croissance dérivé des plaquettes
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Endothélium vasculaire
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Cellules cultivées
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Chimie
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Artère carotide commune
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Biologie cellulaire
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Résistance au cisaillement
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Métabolisme
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Biomedical Engineering
Année:
2005
Type:
Article