Collagen synthesis and expression of connective tissue growth factor in the cultured fibroblasts of human hypertrophic scar / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 124-127, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-327291
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of connective tissue growth factor on the pathogenesis of human hypertrophic scar.</p><p><b>METHODS</b>Normal skin and hypertrophic scar fibroblasts were cultured in vitro. The collagen synthesis of fibroblasts were measured by H3-proline incorporation method. The expression of connective tissue growth factor protein and mRNA of fibroblasts were detected with immunocytochemistry staining and reverse transcription polymerase chain reaction methods.</p><p><b>RESULTS</b>Compared with normal skin fibroblast, the collagen synthesis and the expression of connective tissue growth factor protein and mRNA in the hypertrophic scar fibroblast was higher (P < 0.01).</p><p><b>CONCLUSION</b>Connective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
ARN messager
/
Immunohistochimie
/
Expression des gènes
/
Cellules cultivées
/
Collagène
/
Cicatrice hypertrophique
/
Protéines précoces immédiates
/
RT-PCR
/
Protéines et peptides de signalisation intercellulaire
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Plastic Surgery
Année:
2004
Type:
Article
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