Gene expression and activities analysis of a new fusion protein (RGD) 3/tTF / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 409-412, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-328014
ABSTRACT
To develop a new fusion protein (RGD)3/tTF for the therapy of the selective thrombosis of tumor blood vessels. The fused gene (RGD) 3/tTF was reconstructed by PCR, was cloned into vector pET22 b(+), and expressed in E. coli BL21 (DE3). The fusion protein was purified through Nickel-affinity chromatography column. The tTF activity of the fusion protein was detected by clotting assay and F X activation assay. The specific binding of (RGD) 3/tTF to alphavbeta3 was analyzed by indirect ELISA. The recombinant plasmid pET22 b(+)/(RGD)3/tTF was obtained and expressed in E. coli BL21 (DE3). The purified fusion protein could induce blood coagulation, activiate F X. The ability of (RGD) 3/tTF binding specifically to alphavbeta3 was increased by 32%, compared with RGD/tTF. A new fusion protein (RGD) 3/tTF was successfully expressed in E. coli BL21 (DE3). The expressed proteins retained tTF activity and showed a higher binding to alphavbeta3 than that of RGD/tTF.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Oligopeptides
/
Pharmacologie
/
Liaison aux protéines
/
Coagulation sanguine
/
Protéines de fusion recombinantes
/
Thromboplastine
/
Test ELISA
/
Facteur Xa
/
Expression des gènes
/
Réaction de polymérisation en chaîne
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2007
Type:
Article
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