Studies on changes of in vitro and in vivo material base of Shaoyao Gancao decoction based on HPLC-DAD-ESI-MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1947-1952, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-328054
ABSTRACT
<p><b>OBJECTIVE</b>To study the changes of the in vitro and in vivo material base of Shaoyao Gancao decoction (SGD) based on HPLC-DAD-ESI-MS, and confirm the migrational constituents in plasma.</p><p><b>METHOD</b>Kormasil C18, (4.6 mm x 250 mm, 5 microm with protection column) was employed; acetonitrile and 0.5% acetic acid/water was used as mobile phase for gradient elution, flow rate was set at 0.8 mL x m min(-1), column temperature was 25 degrees C, and methanol was used to remove the protein. A LCQdeca mass spectrometer system equipped with an electrospray ionization iontrap source was used as the detector and operated in the positive ion mode. Ions was scanned from the m/z 100 to m/z 1000, and characteristic ion were secondary schizolysised to obtain data of second order MS.</p><p><b>RESULT</b>The main constitutes of SGD were peoniflorin, Catechin-5-O-glucoside, albiflorin, liquiritin and glycyrrhizic acid etc, and glucosides were found in the plasma samples of rats administered with SGD, which were glucuronide conjunctions of liquiritigenin and isoliquiritigenin.</p><p><b>CONCLUSION</b>Liquid chromatography-mass spectrometry technology provides a convenient and high speed method to analyze the migrational constituents of plasma of rats administered with SGD, therefore, it has a good value in studies on the changes in the material base of traditional Chinese medicine (TCM).</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Analyse chimique du sang
/
Médicaments issus de plantes chinoises
/
Pharmacocinétique
/
Chromatographie en phase liquide à haute performance
/
Rat Sprague-Dawley
/
Spectrométrie de masse ESI
/
Méthodes
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2010
Type:
Article
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