Identification of heplipin inducing apoptosis-related genes in KG-1 human leukemia cells by differential display RT-PCR / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 22-26, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-328378
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether Heplipin can induce KG-1 cell apoptosis and explore apoptosis related differentially expressed genes in KG-1 leukemia cell before and after Heplipin induction.</p><p><b>METHODS</b>DNA distribution and DNA electrophoresis were used to prove that Heplipin can induce KG-1 cell apoptosis. The differential display reverse transcription-polymerase chain reaction (DDRT-PCR) was adopted to screen differentially expressed genes before and after Heplipin induction of KG-1 cells for 16 hours and 20 hours. The differentially expressed genes were cloned and analyzed.</p><p><b>RESULTS</b>Heplipin could induce KG-1 cell apoptosis. There were differentially expressed genes in KG-1 cells before and after induction. Wnt13 and ATPase 3 were apoptosis related differentially downregulated genes after Heplipin induction. Conclusion Heplipin can induce KG-1 cell apoptosis. Heplipin induced KG-1 cell apoptosis is related with Wntl3 and ATPase3 (PSMC3). It is the first report that Wnt13 was detected in leukemia cell line.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
ARN messager
/
Leucémies
/
Apoptose
/
Analyse de profil d'expression de gènes
/
Lignée cellulaire tumorale
/
Proteasome endopeptidase complex
/
Protéines de type Wingless
/
Acides gras insaturés
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2007
Type:
Article
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