Sorting of lymphatic endothelial progenitor cells from canine peripheral blood and their differentiation induction towards endothelial cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 169-173, 2007.
Article
de Zh
| WPRIM
| ID: wpr-328390
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the biological properties of CD34+/CD133 +/VEGFR-3 + lymphatic endothelial progenitor cells in peripheral blood and explore the effects of the VEGF-C/VEGFR-3 signaling pathway on differentiation of lymphatic endothelial progenitor cells to lymphatic endothelial cells.</p><p><b>METHODS</b>Mononuclear cells were isolated from peripheral blood by density centrifugation with Percoll solution, and VEGFR-3+ cells were sorted from them with flow cytometry. Differentiation of VEGFR-3+ cells was induced with VEGF-C. The morphology and ultrastructures of the cells were observed with scanning and transmission electron microscopes. Expression of surface markers were examined with a confocal laser scanning microscope.</p><p><b>RESULTS</b>VEGFR-3+ cells expressed CD34 and CD133 antigen. The percentage of CD34+/ VEGFR-3+ and VEGFR-3+/CD133+ cells were 0.13% and 0.08% of peripheral blood MNC respectively. The size of CD34+/CD133+/VEGFR-3+ cells was about 15 microm in the diameter. After induction with VEGFC, they were increased. The cells were shuttle-like in shape and extended the lamellipodia and many filopodia. After 1 week induction with VEGF-C, they expressed coagulation factor VII related antigen, and at 2 week induction, they showed caveolae on the surface and Weibel-Palade body inside the cells. The specific lymphatic endothelial marker LYVE-1 was expressed on the cells, and no longer expressed CD133.</p><p><b>CONCLUSIONS</b>CD34+/CD133+/VEGFR-3+ lymphatic endothelial progenitor cells from peripheral blood may differentiate into lymphatic endothelial cells. The VEGF-C/VEGFR-3 signaling pathway has important effects on the differentiation of the lymphatic endothelial progenitor cells.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Peptides
/
Pharmacologie
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Cellules souches
/
Glycoprotéines
/
Antigènes CD
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Différenciation cellulaire
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Cellules cultivées
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Antigènes CD34
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Biologie cellulaire
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Récepteur-3 au facteur croissance endothéliale vasculaire
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Hematology
Année:
2007
Type:
Article