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Effects of interleukin 13 on the differentiation and expression of transcription factor c-fos of HEL cells / 中华血液学杂志
Chinese Journal of Hematology ; (12): 538-542, 2006.
Article Dans Chinois | WPRIM | ID: wpr-328422
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of IL-13 on the differentiation and expression of transcription factor c-fos of human erythroleukemia cell line (HEL) cells.</p><p><b>METHODS</b>Reverse transcription polymerase chain reaction (RT-PCR) was used to observe the mRNA expression of IL-13 receptor a 1, GP i b, vWF and c-fos, and Western blot and cytometry were used to analyse their protein expression.</p><p><b>RESULTS</b>IL-13 receptor a 1 was expressed on HEL cells. IL-13 (100 ng/ml ) up-regulated the mRNA expression of GP II b and vWF. The ratio of luminous absorption (LA) of GP I b to p-actin bands ( AB) was 1. 303 in control group, whereas was 2. 912 in experiment group; being 2. 23-fold higher than that in control group (P < 0. 05). The ratio of LA to AB for vWF was 0.217 in control group, and 0. 506 in experiment group; indicating a 2. 33-fold increase in experiment group (P <0. 05). The protein expression of GP I b and vWF was significantly increased in experiment group, compared with that in control group. IL-13 inducing the increased expression of c-fos mRNA and protein of HEL cells peaked at 30 min and 60 min, respectively. The ratio of LA to AB for c-fos was also increased at 30 min and 60 min (P <0. 05).</p><p><b>CONCLUSION</b>IL-13 prompts the differentiation of HEL cells and up-regulates the expression of c-fos.</p>
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pharmacologie / ARN messager / Facteur de von Willebrand / Leucémie érythroblastique aigüe / Différenciation cellulaire / Régulation positive / Protéines proto-oncogènes c-fos / Interleukine-13 / RT-PCR / Glycoprotéine-IIb de membrane plaquettaire Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Hematology Année: 2006 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pharmacologie / ARN messager / Facteur de von Willebrand / Leucémie érythroblastique aigüe / Différenciation cellulaire / Régulation positive / Protéines proto-oncogènes c-fos / Interleukine-13 / RT-PCR / Glycoprotéine-IIb de membrane plaquettaire Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Hematology Année: 2006 Type: Article