Induction of apoptosis of leukemic tumor cells in mouse model with G-quadruplex ligand Tel03 / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 57-60, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-328572
ABSTRACT
This study was aimed to investigate the anti-leukemia activity of Tel03 in vivo. The K562 xenografted leukemia model was established and mice were divided randomly into three groups. Mice of different group were treated with PBS (control), 5 mg/kg Tel03 or 15 mg/kg Tel03 (ip, twice a week) respectively. Tumor volume, body weight and other behavior were observed regularly. Cell apoptosis was detected with TUNEL assay and the expression levels of Bcl-2 and Bax were detected by Western blot. The results indicated that Tel03 exerted anti-leukemia activity in mouse model. Tel03 significantly reduced tumor volume in Tel03-treated group compared with control. In addition, 5 mg/kg Tel03 induced cell apoptosis without exerting apparent toxicity in mice. After Tel03 treatment, the expression of Bcl-2 was inhibited, however, the expression of Bax was up-regulated. It is concluded that G-quadruplex ligand Tel03 can induce cell apoptosis in leukemia mouse model, and this agent may be a potential anticancer drug.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines proto-oncogènes
/
Apoptose
/
Protéines proto-oncogènes c-bcl-2
/
Cellules K562
/
Tests d'activité antitumorale sur modèle de xénogreffe
/
Protéine Bax
/
G-quadruplexes
/
Métabolisme
/
Souris de lignée BALB C
/
Souris nude
Limites du sujet:
Animaux
/
Femelle
/
Humains
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2010
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS