Experimental study of plasmid TGF-beta 1 DNA gene transfer with lipofectamine into rabbit corneal epithelial cells in vitro / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 62-65, 2002.
Article
Dans Anglais
| WPRIM
| ID: wpr-329129
ABSTRACT
To investigate whether the TGF-beta 1 plasmid DNA carried by lipofectamine could be introduced into cultured rabbit corneal epithelial cells, specific expression of the plasmid pMAM TGF-beta 1 in the cultured corneal epithelial cells was studied. Two days after 12 h of transfection of pMAMT-GF-beta 1 mediated by lipofectamine into the cultured corneal epithelial cells, the TGF-beta 1 protein expression specific for pMAMTGF-beta 1 in the cells was detected by means of immunohistochemical staining and the positive rate was 23.37%. The results suggested that foreign plasmid DNA could be effectively delivered into cultured rabbit corneal epithelial cells by means of lipofectamine, and this will provide a promising method of studying TGF-beta 1 on the mechanism of physiology and pathology concerned with corneal epithelial cells.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Plasmides
/
Division cellulaire
/
Cellules cultivées
/
Facteur de croissance transformant bêta
/
Techniques de transfert de gènes
/
Épithélium antérieur de la cornée
/
Biologie cellulaire
/
Facteur de croissance transformant bêta-1
/
Vecteurs génétiques
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Année:
2002
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS