Study of rat osteoblasts transfected by transforming growth factor beta 1 gene / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 116-143, 2002.
Article
Dans Anglais
| WPRIM
| ID: wpr-329167
ABSTRACT
In order to investigate the effect of TGF beta 1 gene transfer on the biological characteristics, the effects of gene transfer and supernatant of transfected osteoblasts on the proliferation and ALP activity of osteoblasts were detected by 3H-TdR and MTT. Our results showed that TGF beta 1 gene transfer had no effect on the biological characteristics and the activated supernatant of transfected osteoblasts stimulated proliferation and inhibited ALP activity of osteoblasts. TGF beta 1 gene transfer could promote the expression of TGF beta 1 and the biological characteristics of transfected osteoblasts were stable, which might be helpful for gene therapy of bone defects in vivo.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoblastes
/
Protéines recombinantes
/
Transfection
/
Division cellulaire
/
Cellules cultivées
/
Facteur de croissance transformant bêta
/
Techniques de transfert de gènes
/
ADN complémentaire
/
Biologie cellulaire
/
Ingénierie tissulaire
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Année:
2002
Type:
Article
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