Non-modified magnetic beads coupled with multiple real-time PCR for detection and quantification of mycotoxigenic fungi in paprika samples / 南方医科大学学报
Journal of Southern Medical University
;
(12): 23-28, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-329180
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for detecting 3 common toxigenic molds (Aspergillus, Penicillium, and Fusarium) based on non-modified magnetic beads coupled with multiple real-time PCR (NMB-multiple qPCR).</p><p><b>METHODS</b>The primers and genus-specific probes were designed based on the rDNA sequences to develop a multiple real-time PCR using non-modified magnetic bead to enrichment of fungal spores. The sensitivity, specificity and repeatability of this assay were evaluated.</p><p><b>RESULTS</b>The detection limit of this assay for spiked samples was 10(4) CFU/g, demonstrating a 10-fold greater detection sensitivity of this assay than that of real-time PCR. The NMB-multiple qPCR assay also showed good specificity and reproducibility and yielded comparable results with those by traditional colony counting method for spiked samples (P>0.05).</p><p><b>CONCLUSION</b>NMB-multiple qPCR assay we established allows rapid and sensitive detection of common mycotoxigenic fungi in paprika.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Penicillium
/
Aspergillus
/
Capsicum
/
Contamination des aliments
/
Reproductibilité des résultats
/
Sensibilité et spécificité
/
Amorces ADN
/
Réaction de polymérisation en chaine en temps réel
/
Phénomènes magnétiques
/
Microbiologie alimentaire
Type d'étude:
Etude diagnostique
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2015
Type:
Article
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