Role of RAGE in lipopolysaccharide-induced cytoskeletal changes in mouse pulmonary microvascular endothelial cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 6-11, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-329183
ABSTRACT
<p><b>OBJECTIVE</b>To investigate lipopolysaccharide (LPS)-induced changes of cytoskeletal filamentous actin in primary isolated pulmonary microvascular endothelial cells (PMVECs) from wild-type and RAGE knock-out mouse.</p><p><b>METHODS</b>The lungs of wild-type and RAGE knock-out mice were digested with collagenase type I to obtain endothelial cells purified by anti-CD31-coupled magnetic beads. The PMVEC identified by factor VIII labeling were stimulated with LPS at different concentrations and the changes of filamentous actin were observed by confocal microscopy.</p><p><b>RESULTS</b>The cultured primary cells showed typical endothelial cell phenotype as examined with factor VIII labeling. LPS stimulation caused rearrangement of the cytoskeletal filament F-actin in wild-type mouse PMVECs with stress fiber formation, but such changes were not obvious in RAGE knock-out mouse PMVECs.</p><p><b>CONCLUSION</b>Mouse PMVECs of a high purity can be obtained by immune magnetic beads. RAGE is involved in LPS-induced destruction of mouse PMVEC cytoskeletons.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phénotype
/
Cytosquelette
/
Récepteurs immunologiques
/
Cellules cultivées
/
Lipopolysaccharides
/
Actines
/
Souris knockout
/
Biologie cellulaire
/
Cellules endothéliales
/
Microvaisseaux
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2015
Type:
Article
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