Detecting MTHFR gene mutation with a new approach: molecular beacons / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 436-437, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-329441
ABSTRACT
<p><b>OBJECTIVE</b>To detect the methylenetetrahydrofolate reductase(MTHFR) gene C677T mutation with molecular beacon technique and assess the revant applicability.</p><p><b>METHODS</b>A total of 228 samples were analyzed using molecular beacons which are oligonucleotide probes to become fluorescent upon hybridization. Wild-type molecular beacon and mutant beacon were designed to detect the genotypes of MTHFR gene.</p><p><b>RESULTS</b>Analysis of the 228 samples indicated that there were three genotypes including 41 homozygous mutants, 113 heterozygous individuals and 74 wild-type individuals. Every sample was identified clearly.</p><p><b>CONCLUSION</b>The present method, a closed-tube PCR/hybridization assay, is a simple, high-throughput and fast procedure that is fully automated for detecting gene mutation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Analyse de mutations d'ADN
/
Sondes oligonucléotidiques
/
Chimie
/
Sensibilité et spécificité
/
Mutation ponctuelle
/
Methylenetetrahydrofolate reductase (NADPH2)
/
Colorants fluorescents
/
Génétique
/
Génotype
/
Méthodes
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2003
Type:
Article
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