Monitoring of pollution of air fine particles (PM2.5) and study on their genetic toxicity / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 452-458, 2004.
Article
Dans Anglais
| WPRIM
| ID: wpr-329615
ABSTRACT
<p><b>OBJECTIVE</b>To compare PM2.5 pollation level between the city of coal-fuel pollution (Taiyuan) and the city of pollution mixed with coal fuels and vehicle exhausts (Beijing), to analyze the concentration of B[a]p and Pb in the pollutants, and to study the DNA damage by PM2.5.</p><p><b>METHODS</b>Air fine particles (PM2.5) were collected in Beijing and Taiyuan by means of the filter membrane method, the concentration of B[a]p and Pb were analyzed by high performance liquid chromatography and atomic absorption spectroscopy respectveily, and the damage of DNA by PM2.5 was detected by single cell gel-electrophoresis (SCGE) using the human lung epithelial cells (A549) as target cells.</p><p><b>RESULTS</b>The concentration of PM2.5 in the winter of Beijing was 0.028-0.436 mg/m(3), and that in Taiyuan was 0.132-0.681 mg/m(3). The concentration of B[a]p was 0.104 and 0.156 microg/mg on PM2.5 of Beijing and Taiyuan, respectively, whereas the concentration of Pb was 1.094 and 1.137 microg/mg on PM2.5 of Beijing and Taiyuan, respectively. Exposure to PM2.5 at the concentrations of 5, 50, and 200 microg/mL for 12 h and 24 h caused DNA damage of the human alveolar epithelium, and the ratios of the tailing and length of the tail were all significantly different from those of the negative control group (P < 0.05), and indicated a dose-response relationship.</p><p><b>CONCLUSION</b>PM2.5 has certain genetic toxicity.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Taille de particule
/
Alvéoles pulmonaires
/
Saisons
/
Benzo[a]pyrène
/
Altération de l'ADN
/
Chine
/
Surveillance de l'environnement
/
Polluants atmosphériques
/
Relation dose-effet des médicaments
/
Effets secondaires indésirables des médicaments
Limites du sujet:
Animaux
/
Humains
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2004
Type:
Article
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