A study on detecting and identifying enteric pathogens with PCR / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 109-120, 2004.
Article
Dans Anglais
| WPRIM
| ID: wpr-329650
ABSTRACT
<p><b>OBJECTIVE</b>To develop a rapid and definite diagnostic test of bacterial enteritis caused by pathogenic enterobacteria, the most frequent etiologic agent of infectious enteritis in the world.</p><p><b>METHODS</b>A set of conventional PCR assays were applied to detect and identify salmonella, shigella, and E. coli O157H7 directly from pure culture and fecal samples. The general primers of pathogenic enterobacteria were located on the uidA gene, which were found not only in E. coli nuclear acid, but also in shigella and salmonella genes. Shigella primer was from ipaH gene whose coded invasive plasmid relative antigen existed both in plasmid and in genome. The primers of salmonella were designed from the 16SrRNA sequence. The primer of E. coli O157H7 was taken from eaeA gene. Five random primers were selected for RAPD. The detection system included common PCR, semi-nested PCR and RAPD.</p><p><b>RESULTS</b>This method was more sensitive, specific and efficient and its processing was rapid and simple. For example, the method could be used to specifically detect and identify salmonella, shigella, and E. coli O157H7, and its sensitivity ranged from 3 to 50 CFU, and its detection time was 4 hours.</p><p><b>CONCLUSION</b>This PCR method, therefore, can serve as a routine and practical protocol for detecting and identifying pathogenic microorganisms from clinical samples.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Salmonella typhi
/
Shigella flexneri
/
ADN bactérien
/
Réaction de polymérisation en chaîne
/
Sensibilité et spécificité
/
Amorces ADN
/
Escherichia coli O157
/
Fèces
/
Microbiologie
Type d'étude:
Etude diagnostique
/
Guide de pratique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2004
Type:
Article
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