Study on the relation between expression of angiotensin II receptor and apoptosis in myocardium in rats of endotoxemia / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 275-279, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-329888
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the expression of angiotensin II (ANG II) receptor and apoptosis in myocardium in rats of endotoxemia.</p><p><b>METHODS</b>Model of endotoxemia was induced by intraperitoneal injection with lipopolysaccharide (LPS) 10 mg/kg in male Wistar rats and saline was injected into control group. The rats were killed at 2 h or 6 h after saline (control) or LPS . Expression of the correlation factors related to apoptosis of Bcl-2, Bax, AT1 and AT2 receptor in myocardial tissue were detected with immunohistochemistry (IHC), and changes of myocardial cells apoptosis was detected by the method of TUNEL. The gene expression of AT1 and AT2 receptor was examined by RT-PCR. The pathological changes of myocardial tissue were observed by electron microscope.</p><p><b>RESULTS</b>Compared with control group , the expression of AT1 and AT2 receptor were significantly decreased, especially in 6 h group; and the expression of Bcl-2 and Bax were decreased, the ratio of Bcl-2/Bax had the downtrend as well as the apoptosis of myocardial cells.</p><p><b>CONCLUSION</b>Interfered by LPS, the down regulation of AT1 and AT2 receptor expression has the negative relation with apoptosis of myocardial cells, this result indicated that down regulation of AT1 and AT2 receptor expression maybe related to cardiac functional impairment, which maybe help us to find a new protective path to prevent myocardial damage induced by systemic inflammatory.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
ARN messager
/
Rat Wistar
/
Apoptose
/
Endotoxémie
/
Biologie cellulaire
/
Myocytes cardiaques
/
Récepteur de type 1 à l'angiotensine-II
/
Récepteur de type 2 à l'angiotensine-II
/
Génétique
/
Métabolisme
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2012
Type:
Article
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