Influence of dexamethasone on fusion of embryonic palatal medial edge epithelium in mouse palatal shelves in vitro / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of dexamethasone on the differentiation and proliferation of type A mouse palatal medial edge epithelial cells when there is type A mouse embryonic palatal mesenchymal cells.</p><p><b>METHODS</b>The mouse palatal shelves were harvested from a female mouse of gestation day 14 by microsurgical dissection and cultured in vitro. The differentiation was investigated through microscope and transmission electron microscope under condition of the palatal shelves fusion.</p><p><b>RESULTS</b>Dexamethasone promoted the palatal medial edge epithelium differentiated into squamause epithelium and affected normal development and obstructed the fusion of mouse palatal shelves.</p><p><b>CONCLUSION</b>The results of histological observation indicate that dexamethasone promotes the proliferation of palatal meseuchymal cells and inhibits the normal differentiation of palatal medial edge epithelial cells, which results in cleft palate.</p>