Molecular identification of hairy antler by analysis of high resolution melting / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 619-623, 2015.
Article
de Zh
| WPRIM
| ID: wpr-330189
Bibliothèque responsable:
WPRO
ABSTRACT
High resolution melting (HRM) , an important technology for genotyping and mutation scanning, has broad prospects in the authenticity of traditional Chinese medicine. This paper selected universal CO I primers and used HRM to establish a new method for authenticity of Hairy Antler. PCR was conducted at the annealing temperature of 60 °C and 45 cycles. The range of the DNA template concentration, the primer concentration and the Mg2+ ion concentration were further optimized. The results showed that the Tm values of Cervus nippon were (81.96 ± 0.07), (84.51 ± 0.03) °C and Cervus elaphus was(82.58 ± 0.13), (85.95 ± 0.05) °C with 10-100 mg · L(-1) DNA template, 0.2 µLmol · L(-1) primer, 2.0 mmol · L(-1) Mg2+. This method can authenticate of hairy antler and is simple, fast, high-throughput, visualization.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Andouillers
/
Normes de référence
/
ADN
/
Cervidae
/
Chimie
/
Réaction de polymérisation en chaîne
/
Classification
/
Amorces ADN
/
Température de transition
/
Génétique
Type d'étude:
Diagnostic_studies
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2015
Type:
Article