HIF-2α/Notch3 pathway mediates CoCl-induced migration and invasion in human breast cancer MCF-7 cells / 生理学报
Acta Physiologica Sinica
;
(6): 783-789, 2016.
Article
Dans Chinois
| WPRIM
| ID: wpr-331603
ABSTRACT
The aim of this study is to investigate the effects of hypoxia inducible factor-2α (HIF-2α) and Notch3 on CoCl-induced migration and invasion of human breast cancer cell line MCF-7. MCF-7 cells were exposed to normoxia (21% O) or chemical hypoxia (21% Oplus CoCl). Short hairpin RNA (shRNA) was used to knock down HIF-2α and Notch3 in MCF-7 cells. The mRNA expression levels of HIF-2α, Notch3 and Hey1 were measured by RT-PCR. Western blot was performed to determine the protein expression levels of HIF-2α, Notch3, Hey1, Snail and E-cadherin. CoCltreatment resulted in higher protein expression levels of HIF-2α, Notch3, Hey1, Snail (P < 0.05) and lower levels of E-cadherin (P < 0.05), and promoted migration and invasion of MCF-7 cells (P < 0.05). shRNA-HIF-2α suppressed CoCl-induced mRNA expression of Notch3 and Hey1. Notch3 knockdown down-regulated Snail and up-regulated E-cadherin at protein level under simulated hypoxia (P < 0.05), and inhibited CoCl-induced migration and invasion of MCF-7 cells (P < 0.05). In conclusion, our data provide evidence that HIF-2α may promote the migration and invasion of MCF-7 cells under chemical hypoxic conditions by potentiating Notch3 pathway.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Tumeurs du sein
/
Transduction du signal
/
Cadhérines
/
Hypoxie cellulaire
/
Régulation négative
/
Régulation positive
/
Mouvement cellulaire
/
Petit ARN interférent
/
Lignée cellulaire tumorale
/
Facteurs de transcription à motif basique hélice-boucle-hélice
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Acta Physiologica Sinica
Année:
2016
Type:
Article
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