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Effects of bushen huoxue recipe on proliferation, differentiation and mineralization of osteoblasts in rats / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 1112-1114, 2005.
Article Dans Chinois | WPRIM | ID: wpr-331775
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of bushen huoxue recipe (BHR) on cell proliferation, differentiation, maturation and mineralization in osteoblasts of rats cultured in vitro.</p><p><b>METHODS</b>Osteoblasts from cranium of newborn SD rats were cultured by collagenase method. MTT, PNPP and ARS were used to observe the proliferation, activity of alkaline phosphatase (ALP) and the formation of mineral node of cultured osteoblasts effected by different concentrations of BHR at different time points (24, 48, 72 hrs after medication).</p><p><b>RESULTS</b>Different concentrations of BHR could enhance the cell proliferation rate and ALP activity, the effects of BHR in high and moderate concentration were more significant in enhancing the cell proliferation rate (P < 0.01). And the number of mineral node of cultured osteoblasts treated by high and moderate concentration BHR was much more than that of the untreated osteoblasts (P < 0.01).</p><p><b>CONCLUSION</b>BHR could promote the proliferation, differentiation, and mineralization of the osteoblasts.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Ostéoblastes / Pharmacologie / Calcification physiologique / Médicaments issus de plantes chinoises / Différenciation cellulaire / Cellules cultivées / Calcium / Rat Sprague-Dawley / Biologie cellulaire / Prolifération cellulaire Limites du sujet: Animaux langue: Chinois Texte intégral: Chinese Journal of Integrated Traditional and Western Medicine Année: 2005 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Ostéoblastes / Pharmacologie / Calcification physiologique / Médicaments issus de plantes chinoises / Différenciation cellulaire / Cellules cultivées / Calcium / Rat Sprague-Dawley / Biologie cellulaire / Prolifération cellulaire Limites du sujet: Animaux langue: Chinois Texte intégral: Chinese Journal of Integrated Traditional and Western Medicine Année: 2005 Type: Article