Effects of emodin on proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line Anip 973 / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 1015-1020, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-331927
ABSTRACT
<p><b>OBJECTIVE</b>To study the suppressive role of emodin on the growth and its effect on the proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line Anip 973.</p><p><b>METHODS</b>The survival rate and the inhibitory rate of Anip 973 cell in vitro were detected by MTT colorimetric assay and cell growth curve assay at different time points under different concentration of emodin; the cell proliferation cycle and the apoptotic rate were examined with flow cytometry analysis, and Caspase-3 protein expression was measured by immunoblotting assay.</p><p><b>RESULTS</b>Emodin inhibited the proliferation of Anip 973 cell at G0/G1 phase, decreased the cell ratio at S phase and activated the Caspase-3 protein. It suppressed the growth of tumor cells and raised the apoptotic rate in a concentration and time depending manner in a certain extent.</p><p><b>CONCLUSION</b>Emodin could suppress the proliferation of Anip 973 cell, and its mechanism of anticancer effect may be through activating Caspase-3, to induce apoptosis and block cell cycle.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Adénocarcinome
/
Cycle cellulaire
/
Technique de Western
/
Émodine
/
Apoptose
/
Lignée cellulaire tumorale
/
Prolifération cellulaire
/
Relation dose-effet des médicaments
Limites du sujet:
Adulte très âgé
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Integrated Traditional and Western Medicine
Année:
2006
Type:
Article
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