Effect of zhigancao decoction containing serum on transient outward potassium current in isolated single ventricular myocyte of rabbits / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of Zhigancao Decoction (ZD) containing serum on transient outward potassium current (I(to)) in isolated single ventricular myocyte of rabbits.</p><p><b>METHODS</b>Single rabbit ventricular myocytes were isolated and divided into 6 groups, the normal group, the non-drug-containing serum group, and the ZD groups, they were treated with normal extracellular fluid alone or with ZD containing serum recording technique was used to observe the changes of I(to).</p><p><b>RESULTS</b>After treated with 5%, 10%, 20% and 40% ZD containing serum, the I(to) was inhibited and its peak value dropped from (16.1 +/- 1.4) PA/PF to (13.9 +/-1.5) PA/PF, (11.8 +/- 1.9) PA/PF, (8.3 +/- 1.5) PA/PF and (8.2 +/- 1.2) PA/PF respectively, and the effect could be eliminated by elution.</p><p><b>CONCLUSION</b>ZD containing serum could inhibit the I(to) in isolated ventricular myocytes of rabbits in a concentration-dependent manner, which may be the mechanism of antiarrhythmic action of ZI).</p>