Nitric oxide regulates c-fos expression in osteoblastic cells induced by wall-shear / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 479-484, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-332120
ABSTRACT
<p><b>OBJECTIVE</b>To observe regulation of nitric oxide on c-fos expression in osteoblastic cells in response to changes in wall-shear stress in vitro.</p><p><b>METHODS</b>Isolated and purified osteoblastic cells from the calvaria of newborn SD rats were cultured and passaged. The third generation cells, pre-treated with 10% FBS DMEM, 0.3 mmol/L L-NMMA DMEM and 0.1 mmol/L SNP DMEM separately, were subjected to wall-shear stress of 1.2 Pa. Gene expression of the c-fos and NOS activity were studied before (0 min) and 10 min, 15 min, 30 min, 60 min after treated with wall-shear stress.</p><p><b>RESULT</b>The expression of c-fos mRNA was increased transiently after application of 1.2 Pa wall-shear stress in osteoblastic cells and peaked at 15 min. The expression of c-fos mRNA was decreased after pre-application with L-NMMA and increased after use of SNP.</p><p><b>CONCLUSION</b>Changes in the osteoblastic cells mechanical environment may cause a dramatic induction of NO and c-fos expression.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoblastes
/
Crâne
/
Contrainte mécanique
/
Phénomènes biomécaniques
/
ARN messager
/
Cellules cultivées
/
Protéines proto-oncogènes c-fos
/
Rat Sprague-Dawley
/
Nitric oxide synthase
/
Biologie cellulaire
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2006
Type:
Article
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