Using 1H-MRS to evaluate the effects of warm ischemia reperfusion injury on the regeneration of hepatic cells in orthotopic transplanted livers in rats / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 274-278, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-332258
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the prognostic value of proton magnetic resonance spectroscopy (1H-MRS) in the study of warm ischemia reperfusion injury to the regeneration of hepatic cells of the livers following their orthotopic transplantation in rats.</p><p><b>METHODS</b>A rat orthotopic liver transplantation (OLT) model with warm ischemia, the experimental group, was established and the same was done with a control group but without warm ischemia of the livers. They were studied at 6 time points (6 hours, 1, 3, 7, 14, and 30 days after OLT). All rats took axial T1 weighted and T2 weighted imaging scans and 1H MR spectroscopies.</p><p><b>RESULTS</b>The positive rate of proliferating cell nuclear antigen (PCNA) and the mean peak choline/water ratio in the experimental group were significantly higher than those in the control group and the peak choline /water ratio had a positive correlation with the positive rate of PCNA. Serum ALT and AST increased significantly after OLT, especially during the 6 hour to 3day period. The levels of ALT and AST were markedly higher in the experimental group compared to the control group.</p><p><b>CONCLUSION</b>Warm ischemia reperfusion injury of OLT has a significant effect on the regeneration of hepatic cells, and the choline peak of 1H-MRS can be used to evaluate the regeneration of hepatic cells non-invasively.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Spectroscopie par résonance magnétique
/
Lésion d'ischémie-reperfusion
/
Transplantation hépatique
/
Rat Sprague-Dawley
/
Hépatocytes
/
Biologie cellulaire
/
Diagnostic
/
Ischémie chaude
/
Survie du greffon
/
Régénération hépatique
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Hepatology
Année:
2008
Type:
Article
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