Effect of endomorphin-1 on hematopoietic regulatory molecules from normal human bone marrow stromal cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1574-1578, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-332316
ABSTRACT
The aim of study was to investigate the regulatory effect of endomorphin-1 (EM-1) on ICAM-1 and VCAM-1 on normal human bone marrow stromal cell surface as well as IL-6 and TNF-α excreted by human normal bone marrow stromal cells. The expression of ICAM-1 and VCAM-1 was determined by flow cytometry, and the concentration of IL-6 and TNF-α was detected by ELISA. The results indicated that the expression of ICAM-1 and VCAM-1 was significantly different from the control group after being cultured with endomorphin-1 for 24 hours (p < 0.05), and different concentrations of endomorphin-1 had no effect on expressions of ICAM-1 and VCAM-1 both; there was no difference in secretion of IL-6 and TNF-α between experimental and control groups (p > 0.05). It is concluded that endomorphin-1 regulates the ICAM-1 and VCAM-1 expression on human normal bone marrow stromal cell surface, but endomorphin-1 does not display any effect on IL-6 and TNF-α secreted by human normal bone marrow stromal cells.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Oligopeptides
/
Pharmacologie
/
Cellules de la moelle osseuse
/
Lignée cellulaire
/
Interleukine-6
/
Facteur de nécrose tumorale alpha
/
Molécule-1 d'adhérence intercellulaire
/
Molécule-1 d'adhérence des cellules vasculaires
/
Biologie cellulaire
/
Cellules souches mésenchymateuses
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2010
Type:
Article
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