Fluorescent microbeads-based multiplex detection of IgM antibodies to pathogens caused viral hemorrhagic fever / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 149-151, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-332399
ABSTRACT
<p><b>OBJECTIVE</b>To develop and evaluate a multiplex detection of IgM antibodies to pathogens caused viral hemorrhagic fever.</p><p><b>METHODS</b>The nucleocapsid proteins (NP) of HTN, SEO, Puu MBV, Lassa, RFV and HPS viruses expressed in prokaryotic cells and purified were coupled to 7 different xMAP fluorescent microbeads. The assay was evaluated and optimized when screened against a panel of reference sera collected from HFRS patients, and compared to commonly used MacELISA Kits.</p><p><b>RESULTS</b>For detection of anti-NP antibodies, the sensitivity and specificity of the assay were comparable to a commonly used MacELISA kit, but it could detect different antigen specific antibodies in one reaction simultaneously.</p><p><b>CONCLUSION</b>A robust, rapid and multiplex assay based on NPs could be developed via Luminex xMAP platform for laboratory diagnosis of viral hemorrhagic fever and seroepidemiological investigation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virologie
/
Virus
/
Sang
/
Immunoglobuline M
/
Dosage immunologique
/
Chimie
/
Protéines nucléocapside
/
Allergie et immunologie
/
Fluorescence
/
Fièvres hémorragiques virales
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2009
Type:
Article
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