Establishment of a fluorescent quantitative PCR detection method for rabies virus and preparation of RNA positive controls / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 504-506, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-332452
ABSTRACT
<p><b>OBJECTIVE</b>Establish the fluorescent quantitative RT-PCR detection method for rabies virus (RV) and construct RNase-resistant virus-like particles as positive controls.</p><p><b>METHODS</b>Analyze the database in GenBank, the probe and the primers were designed in the conservative region of N gene of rabies virus and the method of real-time fluorescent quantitative PCR was obtained; On the basis of MS2 phage, with the technology of gene recombination, prepare the RNase-resistant virus-like particles for RV positive controls;</p><p><b>RESULTS</b>RNase-resistant virus-like particles were obtained after prokaryotic expression in E. coli. The designed primers and probe were confirmed to be very specific and conservative, and be sensitive to-concentration of 15 copies/microl.</p><p><b>CONCLUSION</b>Established the method of detecting rabies virus by reverse transcription real-time quantitative PCR,obtained the RNase-resistant and no infectivity virus-like particles as positive controls of rabies virus.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Rage (maladie)
/
Virus de la rage
/
Pancreatic ribonuclease
/
Virologie
/
ADN viral
/
ARN viral
/
Sondes d'ADN
/
Chimie
/
Charge virale
/
RT-PCR
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2008
Type:
Article
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