Evaluation of Direct Immunofluorescence Test with PCR for Detection of Novel Influenza A (H1N1) Virus during 2009 Pandemic
Yonsei Medical Journal
;
: 680-682, 2011.
Article
Dans Anglais
| WPRIM
| ID: wpr-33249
ABSTRACT
During the 2009 novel influenza (H1N1) pandemic, the sensitivity of direct immunofluorescence assay (DFA) for H1N1 infection was 62% (266/429) of that of the polymerase chain reaction (PCR) test. The sensitivity of the DFA differed significantly with the age of patients the sensitivity was the highest (71.8%) for patients aged or =30 years. The sensitivity of DFA in patients aged > or =30 years was 40.7%. Furthermore, the sensitivity (67.3%, 171/254) of DFA was higher for patients who had a high temperature at admission. An increase in the incidence of H1N1 infection did not influence the sensitivity of DFA (62.1% vs. 62%; p=0.984) test, but resulted in a decrease in the negative predictive value, from 92.4% (700/757) to 69.6% (247/355). PCR may be useful as the initial test for diagnosing H1N1 infection in patients aged > or =30 years with a normal temperature at presentation.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Température du corps
/
Réaction de polymérisation en chaîne
/
Sensibilité et spécificité
/
Facteurs âges
/
Technique d'immunofluorescence directe
/
Grippe humaine
/
Sous-type H1N1 du virus de la grippe A
/
Pandémies
/
Fièvre
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Adolescent
/
Adulte
/
Enfant
/
Humains
langue:
Anglais
Texte intégral:
Yonsei Medical Journal
Année:
2011
Type:
Article
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