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Liver X receptor agonist T0901317 inhibits TGF-β1-induced α-SMA expression in normal human lung fibroblasts / 南方医科大学学报
Journal of Southern Medical University ; (12): 744-748, 2011.
Article Dans Chinois | WPRIM | ID: wpr-332558
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of liver X receptor agonist T0901317 on transforming growth factor-β1 (TGF-β1)-induced expression of α-smooth muscle actin (α-SMA) in normal human lung fibroblasts.</p><p><b>METHODS</b>Primary normal human lung fibroblast isolated from the lung specimens of lung cancer patients by explant culture technique were identified with immunostaining for vimentin and keratin. The cells in passages 4 to 10 were treated with T0901317 and/or TGF-β1, and RT-PCR, Western blotting and immunofluorescence assay were used to detect α-SMA expression in the fibroblasts.</p><p><b>RESULTS</b>Lung fibroblast expressed vimentin but not keratin. The results of RT-PCR, Western blotting and immunofluorescence assay all showed that normal human lung fibroblasts constitutively expressed α-SMA under baseline condition, and TGF-β1 at 5 ng/ml induced a significant upregulation of α-SMA both at the mRNA and protein levels. Liver X receptor agonist T0901317 (5 µg/ml) significantly inhibited TGF-β1-induced upregulation of α-SMA expression.</p><p><b>CONCLUSION</b>Liver X receptor agonist T0901317 can inhibit the upregulation of α-SMA in normal human lung fibroblasts induced by TGF-β1, suggesting the potential value of liver X receptor agonist in the treatment of lung fibrosis.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pharmacologie / Sulfonamides / ARN messager / Cellules cultivées / Actines / Biologie cellulaire / Facteur de croissance transformant bêta-1 / Récepteurs nucléaires orphelins / Fibroblastes / Récepteurs hépatiques X Type d'étude: Étude pronostique Limites du sujet: Femelle / Humains langue: Chinois Texte intégral: Journal of Southern Medical University Année: 2011 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pharmacologie / Sulfonamides / ARN messager / Cellules cultivées / Actines / Biologie cellulaire / Facteur de croissance transformant bêta-1 / Récepteurs nucléaires orphelins / Fibroblastes / Récepteurs hépatiques X Type d'étude: Étude pronostique Limites du sujet: Femelle / Humains langue: Chinois Texte intégral: Journal of Southern Medical University Année: 2011 Type: Article