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Identification of ubiquitously expressed transcript as the potential interactor of hepatitis B virus polymerase / 南方医科大学学报
Journal of Southern Medical University ; (12): 686-689, 2011.
Article Dans Chinois | WPRIM | ID: wpr-332574
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the function of hepatitis B virus polymerase (HBV Pol) in the viral life cycle by screening the proteins interacting with HBV polymerase.</p><p><b>METHODS</b>The HBV Pol gene was constructed into the pGBKT7 vector. GAL4 yeast two-hybrid system was used to screen the human liver cDNA library to obtain proteins which interacted with HBV Pol. GST-pull down assay was applied to confirm the protein interactions.</p><p><b>RESULTS</b>Ubiquitously expressed transcript (UXT) was selected by the yeast two-hybrid system. GST-pull down assay confirmed the in vitro interaction between HBV Pol and UXT.</p><p><b>CONCLUSIONS</b>UXT is a potential interactor of HBV Pol, and this protein interaction may provide clues of the function of HBV Pol in HBV life cycle.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Réplication virale / Produits du gène pol / Virus de l&apos;hépatite B / Techniques de double hybride / Cartographie d&apos;interactions entre protéines / Métabolisme / Protéines tumorales Type d'étude: Etude diagnostique Limites du sujet: Humains langue: Chinois Texte intégral: Journal of Southern Medical University Année: 2011 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Réplication virale / Produits du gène pol / Virus de l&apos;hépatite B / Techniques de double hybride / Cartographie d&apos;interactions entre protéines / Métabolisme / Protéines tumorales Type d'étude: Etude diagnostique Limites du sujet: Humains langue: Chinois Texte intégral: Journal of Southern Medical University Année: 2011 Type: Article