Reciprocal action between BMP-2 and BMP-3 in cultured fibroblast in vitro / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 3-7, 2003.
Article
Dans Anglais
| WPRIM
| ID: wpr-332927
ABSTRACT
<p><b>OBJECTIVE</b>To explore reciprocal action between BMP-2 (bone morphogenetic protein-2) and BMP-3 for better understanding of the mechanism of BMP during bone fracture union.</p><p><b>METHODS</b>rhBMP-2 was added into the cultured fibroblasts with the concentration of 1,200 ng/ml. The expression of BMP-3 in fibroblasts was detected by immunohistochemistry. Eukaryotic expression vector pcDNA3-BMP-3 was transfected into the fibroblasts. After the effective expression of BMP-3 was identified, BMP-2 was also detected by immunohistochemistry in BMP-3 expression cells. The fibroblasts transfected with empty vector pcDNA3 were used as the control.</p><p><b>RESULTS</b>Exogenous rhBMP-2 could promote the expression of BMP-3 in fibroblasts. BMP-3 also could be detected in these cells.</p><p><b>CONCLUSIONS</b>BMP-2 and BMP-3 could reciprocally adjust the expression in fibroblasts.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéogenèse
/
Physiologie
/
Immunohistochimie
/
Cellules cultivées
/
Facteur de croissance transformant bêta
/
Consolidation de fracture
/
Protéines morphogénétiques osseuses
/
Protéine morphogénétique osseuse de type 2
/
Protéine morphogénétique osseuse de type 3
/
Fibroblastes
Type d'étude:
Étude pronostique
langue:
Anglais
Texte intégral:
Chinese Journal of Traumatology
Année:
2003
Type:
Article
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