Design and rapid evaluation of a TaqMan assay for the detection of influenza A viruses / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 80-83, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-333042
ABSTRACT
<p><b>OBJECTIVE</b>To design and rapidly evaluate a TaqMan assay for detecting influenza A viruses.</p><p><b>METHODS</b>The probe and the primers of the assay were designed with the software packages of DNA Star and Primer Premier 5.0. Their specificity and conservation were verified through Blast in GenBank and electronic hybridization. The assay's sensitivity was compared with the standard RT-PCR.</p><p><b>RESULTS</b>The designed primers and probe were confirmed to be very specific and conserved. The assay was 3-27 folds more sensitive than the standard RT-PCR. The RT and PCR steps could be simplified into one step.</p><p><b>CONCLUSION</b>The TaqMan Real-time PCR assay is specific, sensitive and easy to perform.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virus de la grippe A
/
Virologie
/
Oiseaux
/
Reproductibilité des résultats
/
Sensibilité et spécificité
/
RT-PCR
/
Diagnostic
/
Grippe humaine
/
Grippe chez les oiseaux
/
Génétique
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2005
Type:
Article
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