Protective effects of crude polysaccharides from Chroogomphus rutilus on SH-SY5Y cells impaired by MPP(+) / 生理学报
Acta Physiologica Sinica
; (6): 210-216, 2013.
Article
de Zh
| WPRIM
| ID: wpr-333114
Bibliothèque responsable:
WPRO
ABSTRACT
The aim of the present study was to investigate the protective effects of crude polysaccharides from Chroogomphus rutilus on dopaminergic neurons impaired by MPP(+). SH-SY5Y cells were pretreated with crude polysaccharides (200, 400 and 800 μg/mL), and then MPP(+) was added to cell medium. After 48 h of incubation, MTT method was used to detect the survival rate of SH-SY5Y cells damaged by MPP(+). Annexin V-FITC staining and flow cytometry were used to detect apoptotic rate. The results showed that pretreating SH-SY5Y cells with crude polysaccharides (400 and 800 μg/mL) increased the survival rates, and reduced the apoptotic rates of SH-SY5Y cells. To rule out the possibility that crude polysaccharides may decrease actual concentration of MPP(+) by direct binding, we washed off crude polysaccharides before MPP(+) addition. Under this experimental condition, MTT results showed the survival rates of the SH-SY5Y cells were still significantly increased by 800 μg/mL crude polysaccharides pretreatment. These results suggest a protective effect of polysaccharides on the SH-SY5Y cells. Most of this protection is contributed by direct action of polysaccharides on the cells, not by binding with MPP(+). It is indicated that the crude polysaccharides from Chroogomphus rutilus can be developed as a potential drug for Parkinson's disease prevention and treatment in the future.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Polyosides
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Basidiomycota
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1-Méthyl-4-phényl-pyridinium
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Chimie
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Apoptose
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Lignée cellulaire tumorale
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Neurones dopaminergiques
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Acta Physiologica Sinica
Année:
2013
Type:
Article