RhoA-Rho kinase signaling pathway mediates adventitial fibroblasts differentiation to myofibroblasts induced by TGF-β1 / 生理学报
Acta Physiologica Sinica
;
(6): 113-121, 2013.
Article
Dans Anglais
| WPRIM
| ID: wpr-333127
ABSTRACT
Vascular adventitial fibroblasts (AF) differentiation to myofibroblasts (MF) is the critical physiopathologic feature of vascular remodeling. This study was to investigate the role of RhoA-Rho kinase signaling pathway in AF differentiation to MF induced by transforming growth factor β1 (TGF-β1). The results showed that TGF-β1 up-regulated total RhoA protein expression and RhoA activity in cultured AF by Western blotting and Rho pull-down assay, respectively. TGF-β1 up-regulated phospho-Myosin phosphatase target subunit (MYPT1, a downstream substrate of Rho kinase) expression without altering Rho kinase protein expression, indicating TGF-β1 induced the enhancement of activity of Rho kinase. Ad-N19RhoA-hrGFP virus infection and Y27632, a specific inhibitor of Rho kinase, dose-dependently inhibited TGF-β1-induced α-SM-actin and Calponin expression, as markers of MF differentiation. In conclusion, the RhoA-Rho kinase pathway is involved in AF differentiation to MF induced by TGF-β1.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Protéines de liaison au calcium
/
Transduction du signal
/
Différenciation cellulaire
/
Régulation positive
/
Cellules cultivées
/
Actines
/
Protéine G RhoA
/
Biologie cellulaire
/
Facteur de croissance transformant bêta-1
langue:
Anglais
Texte intégral:
Acta Physiologica Sinica
Année:
2013
Type:
Article
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