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Vimentin significantly promoted gallbladder carcinoma metastasis / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 4236-4244, 2011.
Article Dans Anglais | WPRIM | ID: wpr-333580
ABSTRACT
<p><b>BACKGROUND</b>The precise molecular mechanisms underlying the gallbladder carcinoma (GBC) metastasis has not been fully elucidated.</p><p><b>METHODS</b>In the present study, metastasis-associated proteins were identified by comparative proteomic analysis. The functional study of the candidate protein vimentin was further investigated. First, a pair of higher and lower metastatic sublines (termed GBC-SD/M3 and GBC-SD, respectively), originated from the same parental cell line, was screened by spontaneous tumorigenicity and metastasis in vivo in animal study and further characterized by metastatic phenotypes analysis in vitro. Subsequently, a proteomic approach comprised two-dimensional gel electrophoresis analysis and mass spectroscopy was used to identify and compare the protein expression patterns between higher metastatic GBC-SD/M3 and lower metastatic GBC-SD cell lines. Then twenty-six proteins were identified.</p><p><b>RESULTS</b>Among the 26 proteins identified, fourteen proteins were up-regulated and 12 proteins were down-regulated in GBC-SD/M3. Vimentin was identified and found to be overexpressed in GBC-SD/M3 as compared with GBC-SD. This result was further confirmed by quantitative PCR and Western blotting analysis. Furthermore, the cell migration and invasion potency of GBC-SD/M3 in vitro was remarkably suppressed after small interference RNA-mediated knockdown of vimentin. Moreover, immunoblot and immunohistochemical analysis on 12 human GBC specimens showed consistently increased vimentin expression in metastases compared with primary tumors.</p><p><b>CONCLUSION</b>Tumor vimentin level may reflect the pathological progression in some GBC and may be a useful marker for predicting tumor metastasis and a therapeutic target for the treatment of GBC patients with metastases.</p>
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Physiologie / Vimentine / Immunohistochimie / Électrophorèse bidimensionnelle sur gel / Mouvement cellulaire / Technique de Western / Spectrométrie de masse MALDI / RT-PCR / Interférence par ARN Limites du sujet: Animaux / Humains langue: Anglais Texte intégral: Chinese Medical Journal Année: 2011 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Physiologie / Vimentine / Immunohistochimie / Électrophorèse bidimensionnelle sur gel / Mouvement cellulaire / Technique de Western / Spectrométrie de masse MALDI / RT-PCR / Interférence par ARN Limites du sujet: Animaux / Humains langue: Anglais Texte intégral: Chinese Medical Journal Année: 2011 Type: Article